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New tools for confocal macroscopy at OCI
Author(s): Paul Constantinou
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Paper Abstract

Light microscopy is a widely used tool in biomedical research. Fluorescence microscopy concentrates on quantitative and qualitative measurements of the fluorescent light emitted from the specimen under study. This is generally done using fluorescent molecules that can be tagged to antibodies, giving specific information about the micro-environment of the sample, i.e. oxygen concentration (tissue hypoxia), and/or to visualize specific structures, such as, tissue morphology (H & E), and blood vessel location (CD31). Biological applications of fluorescence microscopy such as imaging cut and stained tissue/tumour sections use specimens that 'overfill' the field of view of standard microscope objectives. An average tumour in these studies is 5-10 mm wide, while microscope objectives range in their field of view from -1 mm down to a few hundred microns, with smaller fields as magnification power increases. This can pose some difficulties for studies that look at the expression of a parameter across the entire specimen.

Paper Details

Date Published: 29 August 2017
PDF: 2 pages
Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 103132Y (29 August 2017); doi: 10.1117/12.2283902
Show Author Affiliations
Paul Constantinou, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 10313:
Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging
John C. Armitage, Editor(s)

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