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Proceedings Paper

A method of combining diffuse reflectance and intrinsic fluorescence for diagnosing coronary atherosclerosis
Author(s): Joseph T. Arendt
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Paper Abstract

Intrinsic fluorescence (IF) spectra, computed from measured fluorescence emission and diffuse reflectance (DR) spectra, are free from distortions by scattering and absorption. Using an original instrument called FastEEM, white light DR and fluorescence emission spectra generated at 11 excitation wavelengths were collected from heart transplant and autopsy cases. IF spectra were extracted by combining DR and fluorescence spectra using a photon migration model. IF spectra were fit to a linear combination of collagen and elastin spectra at 342 nm excitation, and collagen and component C at 480 nm. C spectrum was derived from multivariate curve resolution analysis and related to ceroid. We calculated contributions of collagen and elastin to IF at 342 and of C at 480 nm, and contribution of beta-carotene absorption to DR. A diagnostic algorithm was derived. Specificity, sensitivity, and validity were verified by leave-one out cross-validation. 110 coronary segments were studied: 22 normal and intimal fibroplasia and 88 atherosclerotic/atheromatous plaques. An algorithm using collagen contribution to IF at 342 nm, contribution of C to IF at 480 nm and that of beta-carotene to DR had sensitivity 95%, specificity 91% and PPV 98%. Fundamental parameters extracted from IF and DR spectra can accurately diagnose atherosclerotic lesions.

Paper Details

Date Published: 29 August 2017
PDF: 3 pages
Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 103132X (29 August 2017); doi: 10.1117/12.2283901
Show Author Affiliations
Joseph T. Arendt, Cleveland Clinic Foundation (United States)

Published in SPIE Proceedings Vol. 10313:
Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging
John C. Armitage, Editor(s)

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