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Proceedings Paper

Three-dimensional reconstruction and visualization of confocal microscopic images formed in backscattered light
Author(s): Barry R. Masters
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Paper Abstract

How can we use visible light to obtain a three-dimensional image of thick, living biological tissues such as the eye or skin? Confocal light microscopy can be used to obtain a stack of optical sections which are stored as digital images. These optical sections can then be processed in a digital computer to form three-dimensional reconstructions. Four-dimensional microscopy is defined as the three-dimensional reconstruction of specimens which are changing with time. At specific time intervals a set of optical sections are collected through the use of a confocal microscope. Each set of optical sections are then transformed into a three-dimensional reconstruction which corresponds to the individual stack of optical sections taken at a given time.

Paper Details

Date Published: 5 August 1993
PDF: 21 pages
Proc. SPIE 10311, Medical Optical Tomography: Functional Imaging and Monitoring, 103110M (5 August 1993); doi: 10.1117/12.2283767
Show Author Affiliations
Barry R. Masters, Uniformed Services Univ. of Health Sciences (United States)


Published in SPIE Proceedings Vol. 10311:
Medical Optical Tomography: Functional Imaging and Monitoring
Gerhard J. Mueller, Editor(s)

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