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Proceedings Paper

An optical fiber-based diffuse reflectance spectrometer for non-invasive investigation of photodynamic sensitizers in vivo
Author(s): Brian C. Wilson
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Paper Abstract

A prototype instrument is described, based on optical fiber light delivery and detection, for quantitative, non-invasive, in vivo studies of photosensitizers used in photodynamic therapy. The primary purpose of the instrument is the measurement of absolute photosensitizer uptake in tissues. This is achieved by measuring the spectrum of diffusely reflected light and identifying the characteristic absorption spectrum of the photosensitizer. This absorbance measurement is converted to equivalent absolute photosensitizer concentration by a procedure which separately determines the intrinsic optical absorption and transport scatter coefficients of the tissue. The procedure involves measuring the radial dependence of the diffusely reflected light on the tissue surface as a function of wavelength. The principles and operation of the instrument have been evaluated in tissue-simulating phantoms and in normal and tumor tissues in animal models in vivo. The outstanding problems and future development of this method, and of analogous quantitative measurements of photosensitizer fluorescence in vivo are discussed.

Paper Details

Date Published: 21 January 1990
PDF: 14 pages
Proc. SPIE 10306, Future Directions and Applications in Photodynamic Therapy, 103060H (21 January 1990); doi: 10.1117/12.2283679
Show Author Affiliations
Brian C. Wilson, McMaster Univ. (Canada)


Published in SPIE Proceedings Vol. 10306:
Future Directions and Applications in Photodynamic Therapy
Charles J. Gomer, Editor(s)

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