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Multimodal fiber-probe spectroscopy as a clinical tool for diagnosing and classifying biological tissues
Author(s): Riccardo Cicchi; Suresh Anand; Riccardo Fantechi; Flavio Giordano; Mauro Gacci; Valerio Conti; Gabriella Nesi; Anna Maria Buccoliero; Marco Carini; Renzo Guerrini; Francesco Saverio Pavone
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Paper Abstract

An optical fiber probe for multimodal spectroscopy was designed, developed and used for tissue diagnostics. The probe, based on a fiber bundle with optical fibers of various size and properties, allows performing spectroscopic measurements with different techniques, including fluorescence, Raman, and diffuse reflectance, using the same probe. Two visible laser diodes were used for fluorescence spectroscopy, a laser diode emitting in the NIR was used for Raman spectroscopy, and a fiber-coupled halogen lamp for diffuse reflectance. The developed probe was successfully employed for diagnostic purposes on various tissues, including brain and bladder. In particular, the device allowed discriminating healthy tissue from both tumor and dysplastic tissue as well as to perform tumor grading. The diagnostic capabilities of the method, determined using a cross-validation method with a leave-one-out approach, demonstrated high sensitivity and specificity for all the examined samples, as well as a good agreement with histopathological examination performed on the same samples. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities with respect to what can be obtained from individual techniques. The experimental setup presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used clinically for guiding surgical resection in the near future.

Paper Details

Date Published: 28 July 2017
PDF: 3 pages
Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics, 104110E (28 July 2017); doi: 10.1117/12.2283278
Show Author Affiliations
Riccardo Cicchi, Istituto Nazionale di Ottica, CNR (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Univ. degli Studi di Firenze (Italy)
Suresh Anand, Istituto Nazionale di Ottica, CNR (Italy)
Riccardo Fantechi, Univ. degli Studi di Firenze (Italy)
Flavio Giordano, Azienda Ospedaliera Univ. Anna Meyer (Italy)
Mauro Gacci, Univ. degli Studi di Firenze (Italy)
Valerio Conti, Azienda Ospedaliera Univ. Anna Meyer (Italy)
Univ. degli Studi di Firenze (Italy)
Gabriella Nesi, Univ. degli Studi di Firenze (Italy)
Anna Maria Buccoliero, Univ. degli Studi di Firenze (Italy)
Marco Carini, Univ. degli Studi di Firenze (Italy)
Renzo Guerrini, Azienda Ospedaliera Univ. Anna Meyer (Italy)
Univ. degli Studi di Firenze (Italy)
Francesco Saverio Pavone, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Univ. degli Studi di Firenze (Italy)


Published in SPIE Proceedings Vol. 10411:
Clinical and Preclinical Optical Diagnostics
J. Quincy Brown; Ton G. van Leeuwen, Editor(s)

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