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Proceedings Paper

Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy
Author(s): Rafay Ahmed; Alan Wing Lun Law; Tsz Wing Cheung; Condon Lau
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Paper Abstract

Subcritical calvarial defects are important to study bone regeneration during healing. In this study 1mm calvarial defects were created using trephine in the parietal bones of Sprague-Dawley rats (n=7) that served as in vivo defects. Subjects were sacrificed after 7 days and the additional defects were created on the harvested skull with the same method to serve as control defects. Raman spectroscopy is established to investigate mineral/matrix ratio, carbonate/phosphate ratio and crystallinity of three different surfaces; in vivo defects, control defects and normal surface. Results show 21% and 23% decrease in mineral/matrix after 7 days of healing from surface to in vivo and control to in vivo defects, respectively. Carbonate to phosphate ratio was found to be increased by 39% while crystallinity decreased by 26% in both surface to in vivo and control to in vivo defects. This model allows to study the regenerated bone without mechanically perturbing healing surface.

Paper Details

Date Published: 1 August 2017
PDF: 4 pages
Proc. SPIE 10417, Medical Laser Applications and Laser-Tissue Interactions VIII, 104170O (1 August 2017); doi: 10.1117/12.2283272
Show Author Affiliations
Rafay Ahmed, City Univ. of Hong Kong (Hong Kong, China)
Alan Wing Lun Law, City Univ. of Hong Kong (Hong Kong, China)
Tsz Wing Cheung, City Univ. of Hong Kong (Hong Kong, China)
Condon Lau, City Univ. of Hong Kong (Hong Kong, China)


Published in SPIE Proceedings Vol. 10417:
Medical Laser Applications and Laser-Tissue Interactions VIII
Lothar D. Lilge, Editor(s)

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