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UbasM: a simple, rapid, efficient balanced optical clearing method for brain imaging
Author(s): Lingling Chen; Guiye Li; Yingchao Li; Lina Liu; Ang Liu; Shuangchen Ruan
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Paper Abstract

Optical clearing methods are highly in demand in organism-level biomedical system research since they can facilitate deep optical imaging by reducing light scattering in tissue and then enable three-dimensional signal visualization and quantification of tissues. While the previously reported optical clearing methods have addressed some of six key issues (i.e. transparency, efficiency, reproducibility, preservation of emission from fluorescence proteins, preservation of membrane integrity, and the ease of operation), none has yet addressed all of them. Here, we present a new, convenient, inexpensive and reproducible approach to optical clearing, termed UbasM, providing unprecedented performance in terms of clearing rate, the ease of operation and satisfactory fluorescence protein/membrane integrity preservation while achieving sufficient transparency to permit 3D volumetric imaging.

Paper Details

Date Published: 12 February 2018
PDF: 5 pages
Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104810W (12 February 2018); doi: 10.1117/12.2283223
Show Author Affiliations
Lingling Chen, Shenzhen Univ. (China)
Guiye Li, Shenzhen Univ. (China)
Yingchao Li, Shenzhen Univ. (China)
Lina Liu, Shenzhen Univ. (China)
Ang Liu, Shenzhen Univ. (China)
Shuangchen Ruan, Shenzhen Univ. (China)


Published in SPIE Proceedings Vol. 10481:
Neural Imaging and Sensing 2018
Qingming Luo; Jun Ding, Editor(s)

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