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Improvements in low-cost label-free QPI microscope for live cell imaging
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Paper Abstract

This paper reports an improvement in the development of a low-cost QPI microscope offering new capabilities in term of phase measurement accuracy for label-free live samples in the longer term (i.e., hours to days). The spatially separated scattered and non-scattered image light fields are reshaped in the Fourier plane and modulated to form an interference image at a CCD camera. The apertures that enable these two beams to be generated have been optimised by means of laser-cut apertures placed on the mirrors of a Michelson interferometer and has improved the phase measuring and reconstruction capability of the QPI microscope. The microscope was tested with transparent onion cells as an object of interest.

Paper Details

Date Published: 28 July 2017
PDF: 9 pages
Proc. SPIE 10414, Advances in Microscopic Imaging, 104140F (28 July 2017); doi: 10.1117/12.2282230
Show Author Affiliations
C. Seniya, The Univ. of Warwick (United Kingdom)
C. E. Towers, The Univ. of Warwick (United Kingdom)
D. P. Towers, The Univ. of Warwick (United Kingdom)


Published in SPIE Proceedings Vol. 10414:
Advances in Microscopic Imaging
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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