Share Email Print
cover

Proceedings Paper • new

Multi-modal microscopy platform including optical tweezers and the need to perform spherical wave vectors decompositions for optical force and signal intensity calculations (Conference Presentation)
Author(s): Carlos Lenz Cesar; Wendel L. Moreira; Antonio A. R. Neves; André A. de Thomaz; Diogo B. Almeida; Adriana Fontes; Hernandes F. Carvalho; Vitor B. Pelegati

Paper Abstract

We will present the multi-modal photonic platform including Optical Tweezers, linear and non linear optics techniques in a single instrument to allow parallel information gathering during single cell processes. The platform includes the following techniques: multipoint Optical Tweezers; Laser cutting; multi/single photon fluorescence, Fluorescence Lifetime Imaging (FLIM); Förster Resonant Energy Transfer (FLIM-FRET); Fluorescence Correlation Spectroscopy (FCS); Raman; Second/Third Harmonic Generation (SHG/THG); Coherent AntiStokes Raman Scattering (CARS) and cascade CARS; Near field tip-enhancement and 1 and 2 photons Photoluminescence Excitation Spectroscopy (1-2 PLE). Next, we will discuss the issue of spherical wave vectors decomposition of any optical beam in the Fourier space without any approximation solving the problem of spherical Bessel functions cancellation in both sides of the expansion. This expansion is the necessary first step to perform optical forces, as well as optical signals intensities, of scattering/absorbing particles. The limit of Rayleigh regime is easily obtained.

Paper Details

Date Published: 21 September 2017
PDF
Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 1034718 (21 September 2017); doi: 10.1117/12.2277331
Show Author Affiliations
Carlos Lenz Cesar, Univ. Federal do Ceara (Brazil)
Instituto de Física "Gleb Wataghin" (Brazil)
Wendel L. Moreira, Petrobras (Brazil)
Antonio A. R. Neves, Univ. Federal do ABC (Brazil)
André A. de Thomaz, UNICAMP (Brazil)
Diogo B. Almeida, Univ. of Michigan (United States)
Adriana Fontes, Univ. Federal de Pernambuco (Brazil)
Hernandes F. Carvalho, Univ. Estadual de Campinas (Brazil)
Vitor B. Pelegati, Univ. Estadual de Campinas (Brazil)


Published in SPIE Proceedings Vol. 10347:
Optical Trapping and Optical Micromanipulation XIV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

© SPIE. Terms of Use
Back to Top