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Proceedings Paper

Optically enhanced acoustophoresis
Author(s): Craig McDougall; Paul O'Mahoney; Alan McGuinn; Nicholas A. Willoughby; Yongqiang Qiu; Christine E. M. Demore; Michael P. MacDonald
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Paper Abstract

Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several strategies for addressing the requirements for high throughput without labeling via: dimensional scaling, rare species targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One such approach can be termed "optically enhanced acoustophoresis" which combines the ability of acoustics to handle large volumes of analyte with the high specificity of optical sorting.

Paper Details

Date Published: 25 August 2017
PDF: 10 pages
Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 103471B (25 August 2017); doi: 10.1117/12.2276323
Show Author Affiliations
Craig McDougall, Univ. of Dundee (United Kingdom)
Paul O'Mahoney, Univ. of Dundee (United Kingdom)
Alan McGuinn, Heriot Watt Univ. (United Kingdom)
Nicholas A. Willoughby, Heriot-Watt Univ. (United Kingdom)
Yongqiang Qiu, Univ. of Glasgow (United Kingdom)
Christine E. M. Demore, Univ. of Toronto (Canada)
Michael P. MacDonald, Univ. of Dundee (United Kingdom)

Published in SPIE Proceedings Vol. 10347:
Optical Trapping and Optical Micromanipulation XIV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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