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Proceedings Paper

Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy
Author(s): Jongho Baek; Jieqiong Lou; Simao Coelho; Yean Jin Lim; Silvia Seidlitz; Philip R. Nicovich; Christian Wunder; Ludger Johannes; Katharina Gaus
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Paper Abstract

Lattice light-sheet (LLS) microscopy provides ultrathin light sheets of a two-dimensional optical lattice that allows us imaging three-dimensional (3D) objects for hundreds of time points at sub-second intervals and at or below the diffraction limit. Galectin-3 (Gal3), a carbohydrate-binding protein, triggers glycosphingolipid (GSL)-dependent biogenesis of morphologically distinct endocytic vesicles that are cargo specific and clathrin independent. In this study, we apply LLS microscopy to study the dynamics of Gal3 dependent endocytosis in live T cells. This will allow us to observe Gal3-mediated endocytosis at high temporal and excellent 3D spatial resolution, which may shed light on our understanding of the mechanism and physiological function of Gal3-induced endocytosis.

Paper Details

Date Published: 29 April 2017
PDF: 4 pages
Proc. SPIE 10340, International Conference on Biophotonics V, 103400J (29 April 2017); doi: 10.1117/12.2275706
Show Author Affiliations
Jongho Baek, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence in Advanced Molecular Imaging (Australia)
Jieqiong Lou, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence in Advanced Molecular Imaging (Australia)
Simao Coelho, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence in Advanced Molecular Imaging (Australia)
Yean Jin Lim, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence in Advanced Molecular Imaging (Australia)
Silvia Seidlitz, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence in Advanced Molecular Imaging (Australia)
Philip R. Nicovich, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence in Advanced Molecular Imaging (Australia)
Christian Wunder, Institut Curie, CNRS, INSERM, PSL Research Univ. (France)
Ludger Johannes, Institut Curie, CNRS, INSERM, PSL Research Univ. (France)
Katharina Gaus, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence in Advanced Molecular Imaging (Australia)


Published in SPIE Proceedings Vol. 10340:
International Conference on Biophotonics V
David D. Sampson; Dennis L. Matthews; Jürgen Popp; Halina Rubinsztein-Dunlop; Brian C. Wilson, Editor(s)

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