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Proceedings Paper

Dielectrophoretic focusing integrated pulsed laser activated cell sorting
Author(s): Xiongfeng Zhu; Yu-Chun Kung; Ting-Hsiang Wu; Michael A. Teitell; Pei-Yu Chiou
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Paper Abstract

We present a pulsed laser activated cell sorter (PLACS) integrated with novel sheathless size-independent dielectrophoretic (DEP) focusing. Microfluidic fluorescence activated cell sorting (μFACS) systems aim to provide a fully enclosed environment for sterile cell sorting and integration with upstream and downstream microfluidic modules. Among them, PLACS has shown a great potential in achieving comparable performance to commercial aerosol-based FACS (>90% purity at 25,000 cells sec−1). However conventional sheath flow focusing method suffers a severe sample dilution issue. Here we demonstrate a novel dielectrophoresis-integrated pulsed laser activated cell sorter (DEP-PLACS). It consists of a microfluidic channel with 3D electrodes laid out to provide a tunnel-shaped electric field profile along a 4cmlong channel for sheathlessly focusing microparticles/cells into a single stream in high-speed microfluidic flows. All focused particles pass through the fluorescence detection zone along the same streamline regardless of their sizes and types. Upon detection of target fluorescent particles, a nanosecond laser pulse is triggered and focused in a neighboring channel to generate a rapidly expanding cavitation bubble for precise sorting. DEP-PLACS has achieved a sorting purity of 91% for polystyrene beads at a throughput of 1,500 particle/sec.

Paper Details

Date Published: 25 August 2017
PDF: 7 pages
Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 103472K (25 August 2017); doi: 10.1117/12.2275569
Show Author Affiliations
Xiongfeng Zhu, Univ. of California, Los Angeles (United States)
Yu-Chun Kung, Univ. of California, Los Angeles (United States)
Ting-Hsiang Wu, NantWorks, LLC (United States)
Michael A. Teitell, Univ. of California, Los Angeles (United States)
Pei-Yu Chiou, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 10347:
Optical Trapping and Optical Micromanipulation XIV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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