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Proceedings Paper

Double emulsion electrospun nanofibers as a growth factor delivery vehicle for salivary gland regeneration
Author(s): Zahraa I. Foraida; Anna Sharikova; Lubna N. Peerzada; Alexander Khmaladze; Melinda Larsen; James Castracane
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Paper Abstract

Sustained delivery of growth factors, proteins, drugs and other biologically active molecules is necessary for tissue engineering applications. Electrospun fibers are attractive tissue engineering scaffolds as they partially mimic the topography of the extracellular matrix (ECM). However, they do not provide continuous nourishment to the tissue. In search of a biomimetic scaffold for salivary gland tissue regeneration, we previously developed a blend nanofiber scaffold composed of the protein elastin and the synthetic polymer polylactic-co-glycolic acid (PLGA). The nanofiber scaffold promoted in vivo-like salivary epithelial cell tissue organization and apicobasal polarization. However, in order to enhance the salivary cell proliferation and biomimetic character of the scaffold, sustained growth factor delivery is needed. The composite nanofiber scaffold was optimized to act as a growth factor delivery system using epidermal growth factor (EGF) as a model protein. The nanofiber/EGF hybrid nanofibers were synthesized by double emulsion electrospinning where EGF is emulsified within a water/oil/water (w/o/w) double emulsion system. Successful incorporation of EGF was confirmed using Raman spectroscopy. EGF release profile was characterized using enzyme-linked immunosorbent assay (ELIZA) of the EGF content. Double emulsion electrospinning resulted in slower release of EGF. We demonstrated the potential of the proposed double emulsion electrospun nanofiber scaffold for the delivery of growth factors and/or drugs for tissue engineering and pharmaceutical applications.

Paper Details

Date Published: 29 August 2017
PDF: 9 pages
Proc. SPIE 10352, Biosensing and Nanomedicine X, 103520E (29 August 2017); doi: 10.1117/12.2275489
Show Author Affiliations
Zahraa I. Foraida, SUNY Polytechnic Institute (United States)
Anna Sharikova, Univ. at Albany (United States)
Lubna N. Peerzada, Univ. at Albany (United States)
Alexander Khmaladze, Univ. at Albany (United States)
Melinda Larsen, Univ. at Albany (United States)
James Castracane, SUNY Polytechnic Institute (United States)


Published in SPIE Proceedings Vol. 10352:
Biosensing and Nanomedicine X
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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