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Proceedings Paper

Ultra-fast 3D scanning and holographic illumination in non-linear microscopy using acousto-optic deflectors
Author(s): Walther Akemann; Cathie Ventalon; Jean-François Léger; Benjamin Mathieu; Stéphane Dieudonné; Baptiste Blochet; Sylvain Gigan; Laurent Bourdieu
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Paper Abstract

Decoding of information in the brain requires the imaging of large neuronal networks using e.g. two-photon microscopy (TPM). Fast control of the focus in 3D can be achieved with phase shaping of the light beam using acoustooptic deflectors (AODs). However, beam shaping using AODs is not straightforward because of non-stationary of acousto-optic diffraction. Here, we demonstrated a new stable AOD-based phase modulator, which operates at a rate of up to about hundred kHz. It provides opportunity for 3D scanning in TPM with the possibility to correct aberrations independently for every focus position or to achieve refocusing of scattered photons in rapidly decorrelating tissues.

Paper Details

Date Published: 18 April 2017
PDF: 3 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510L (18 April 2017); doi: 10.1117/12.2275143
Show Author Affiliations
Walther Akemann, Ecole Normale Supérieure, IBENS, CNRS, INSERM (France)
Cathie Ventalon, Ecole Normale Supérieure, IBENS, CNRS, INSERM (France)
Jean-François Léger, Ecole Normale Supérieure, IBENS, CNRS, INSERM (France)
Benjamin Mathieu, Ecole Normale Supérieure, IBENS, CNRS, INSERM (France)
Stéphane Dieudonné, Ecole Normale Supérieure, IBENS, CNRS, INSERM (France)
Baptiste Blochet, Ecole Normale Supérieure, IBENS, CNRS, INSERM (France)
Lab. Kastler Brossel, ENS-PSL Research Univ., CNRS, UPMC-Sorbonnes Univ. (France)
Sylvain Gigan, Lab. Kastler Brossel, ENS-PSL Research Univ., CNRS, UPMC-Sorbonnes Univ. (France)
Laurent Bourdieu, Ecole Normale Supérieure, IBENS, CNRS, INSERM (France)


Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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