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Proceedings Paper

Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons
Author(s): Tatsunori Kishimoto; Yasuyo Maezawa; Suguru N. Kudoh; Takahisa Taguchi; Chie Hosokawa
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Paper Abstract

Molecular dynamics of glutamate receptor, which is major neurotransmitter receptor at excitatory synapse located on neuron, is essential for synaptic plasticity in the complex neuronal networks. Here we studied molecular dynamics in an optical trap of AMPA-type glutamate receptor (AMPAR) labeled with quantum-dot (QD) on living neuronal cells with fluorescence imaging and fluorescence correlation spectroscopy (FCS). When a 1064-nm laser beam for optical trapping was focused on QD-AMPARs located on neuronal cells, the fluorescence intensity of QD-AMPARs gradually increased at the focal spot. Using single-particle tracking of QD-AMPARs on neurons, the average diffusion coefficient decreased in an optical trap. Moreover, the decay time obtained from FCS analysis increased with the laser power and the initial assembling state of AMPARs depended on culturing day, suggesting that the motion of QD-AMPAR was constrained in an optical trap.

Paper Details

Date Published: 18 April 2017
PDF: 2 pages
Proc. SPIE 10252, Optical Manipulation Conference, 1025208 (18 April 2017); doi: 10.1117/12.2275026
Show Author Affiliations
Tatsunori Kishimoto, National Institute of Advanced Industrial Science and Technology (Japan)
Kwansei Gakuin Univ. (Japan)
Yasuyo Maezawa, National Institute of Advanced Industrial Science and Technology (Japan)
Suguru N. Kudoh, Kwansei Gakuin Univ. (Japan)
Takahisa Taguchi, National Institute of Information and Communications Technology (Japan)
Chie Hosokawa, National Institute of Advanced Industrial Science and Technology (Japan)

Published in SPIE Proceedings Vol. 10252:
Optical Manipulation Conference
Takashige Omatsu, Editor(s)

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