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Proceedings Paper

Orientational imaging of a single plasmonic nanoparticle using dark-field hyperspectral imaging
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Paper Abstract

Orientation of plasmonic nanostructures is an important feature in many nanoscale applications such as catalyst, biosensors DNA interactions, protein detections, hotspot of surface enhanced Raman spectroscopy (SERS), and fluorescence resonant energy transfer (FRET) experiments. However, due to diffraction limit, it is challenging to obtain the exact orientation of the nanostructure using standard optical microscope. Hyperspectral Imaging Microscopy is a state-of-the-art visualization technology that combines modern optics with hyperspectral imaging and computer system to provide the identification and quantitative spectral analysis of nano- and microscale structures. In this work, initially we use transmitted dark field imaging technique to locate single nanoparticle on a glass substrate. Then we employ hyperspectral imaging technique at the same spot to investigate orientation of single nanoparticle. No special tagging or staining of nanoparticle has been done, as more likely required in traditional microscopy techniques. Different orientations have been identified by carefully understanding and calibrating shift in spectral response from each different orientations of similar sized nanoparticles. Wavelengths recorded are between 300 nm to 900 nm. The orientations measured by hyperspectral microscopy was validated using finite difference time domain (FDTD) electrodynamics calculations and scanning electron microscopy (SEM) analysis. The combination of high resolution nanometer-scale imaging techniques and the modern numerical modeling capacities thus enables a meaningful advance in our knowledge of manipulating and fabricating shaped nanostructures. This work will advance our understanding of the behavior of small nanoparticle clusters useful for sensing, nanomedicine, and surface sciences.

Paper Details

Date Published: 25 August 2017
PDF: 6 pages
Proc. SPIE 10346, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XV, 1034631 (25 August 2017); doi: 10.1117/12.2274695
Show Author Affiliations
Nishir Mehta, Louisiana State Univ. (United States)
Amirreza Mahigir, Louisiana State Univ. (United States)
Georgios Veronis, Louisiana State Univ. (United States)
Manas Ranjan Gartia, Louisiana State Univ. (United States)

Published in SPIE Proceedings Vol. 10346:
Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XV
Din Ping Tsai; Takuo Tanaka, Editor(s)

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