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Proceedings Paper

Imaging cellular and subcellular structure of human brain tissue using micro computed tomography
Author(s): Anna Khimchenko; Christos Bikis; Gabriel Schweighauser; Jürgen Hench; Alexandra-Teodora Joita-Pacureanu; Peter Thalmann; Hans Deyhle; Bekim Osmani; Natalia Chicherova; Simone E. Hieber; Peter Cloetens; Magdalena Müller-Gerbl ; Georg Schulz; Bert Müller
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Paper Abstract

Brain tissues have been an attractive subject for investigations in neuropathology, neuroscience, and neurobiol- ogy. Nevertheless, existing imaging methodologies have intrinsic limitations in three-dimensional (3D) label-free visualisation of extended tissue samples down to (sub)cellular level. For a long time, these morphological features were visualised by electron or light microscopies. In addition to being time-consuming, microscopic investigation includes specimen fixation, embedding, sectioning, staining, and imaging with the associated artefacts. More- over, optical microscopy remains hampered by a fundamental limit in the spatial resolution that is imposed by the diffraction of visible light wavefront. In contrast, various tomography approaches do not require a complex specimen preparation and can now reach a true (sub)cellular resolution. Even laboratory-based micro computed tomography in the absorption-contrast mode of formalin-fixed paraffin-embedded (FFPE) human cerebellum yields an image contrast comparable to conventional histological sections. Data of a superior image quality was obtained by means of synchrotron radiation-based single-distance X-ray phase-contrast tomography enabling the visualisation of non-stained Purkinje cells down to the subcellular level and automated cell counting. The question arises, whether the data quality of the hard X-ray tomography can be superior to optical microscopy. Herein, we discuss the label-free investigation of the human brain ultramorphology be means of synchrotron radiation-based hard X-ray magnified phase-contrast in-line tomography at the nano-imaging beamline ID16A (ESRF, Grenoble, France). As an example, we present images of FFPE human cerebellum block. Hard X-ray tomography can provide detailed information on human tissues in health and disease with a spatial resolution below the optical limit, improving understanding of the neuro-degenerative diseases.

Paper Details

Date Published: 25 September 2017
PDF: 12 pages
Proc. SPIE 10391, Developments in X-Ray Tomography XI, 103910K (25 September 2017); doi: 10.1117/12.2274120
Show Author Affiliations
Anna Khimchenko, Univ. Basel (Switzerland)
Christos Bikis, Univ. Basel (Switzerland)
Gabriel Schweighauser, Univ. Hospital Basel (Switzerland)
Jürgen Hench, Univ. Hospital Basel (Switzerland)
Alexandra-Teodora Joita-Pacureanu, ESRF - The European Synchrotron (France)
Peter Thalmann, Univ. Basel (Switzerland)
Hans Deyhle, Univ. Basel (Switzerland)
Bekim Osmani, Univ. Basel (Switzerland)
Natalia Chicherova, Univ. Basel (Switzerland)
Simone E. Hieber, Univ. Basel (Switzerland)
Peter Cloetens, ESRF - The European Synchrotron (France)
Magdalena Müller-Gerbl , Univ. Basel (Switzerland)
Georg Schulz, Univ. Basel (Switzerland)
Bert Müller, Univ. Basel (Switzerland)


Published in SPIE Proceedings Vol. 10391:
Developments in X-Ray Tomography XI
Bert Müller; Ge Wang, Editor(s)

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