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Proceedings Paper

Assessment of imaging quality in magnified phase CT of human bone tissue at the nanoscale
Author(s): Boliang Yu; Max Langer; Alexandra Pacureanu; Remy Gauthier; Helene Follet; David Mitton; Cecile Olivier; Peter Cloetens; Francoise Peyrin
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Paper Abstract

Bone properties at all length scales have a major impact on the fracture risk in disease such as osteoporosis. However, quantitative 3D data on bone tissue at the cellular scale are still rare. Here we propose to use magnified X-ray phase nano-CT to quantify bone ultra-structure in human bone, on the new setup developed on the beamline ID16A at the ESRF, Grenoble. Obtaining 3D images requires the application of phase retrieval prior to tomographic reconstruction. Phase retrieval is an ill-posed problem for which various approaches have been developed. Since image quality has a strong impact on the further quantification of bone tissue, our aim here is to evaluate different phase retrieval methods for imaging bone samples at the cellular scale. Samples from femurs of female donors were scanned using magnified phase nano-CT at voxel sizes of 120 and 30 nm with an energy of 33 keV. Four CT scans at varying sample-to-detector distances were acquired for each sample. We evaluated three phase retrieval methods adapted to these conditions: Paganin’s method at single distance, Paganin’s method extended to multiple distances, and the contrast transfer function (CTF) approach for pure phase objects. These methods were used as initialization to an iterative refinement step. Our results based on visual and quantitative assessment show that the use of several distances (as opposed to single one) clearly improves image quality and the two multi-distance phase retrieval methods give similar results. First results on the segmentation of osteocyte lacunae and canaliculi from such images are presented.

Paper Details

Date Published: 3 October 2017
PDF: 7 pages
Proc. SPIE 10391, Developments in X-Ray Tomography XI, 103910L (3 October 2017); doi: 10.1117/12.2272561
Show Author Affiliations
Boliang Yu, Univ. Lyon, INSA Lyon, Univ. Claude Bernard Lyon 1, Univ. Jean Monnet Saint Etienne, CREATIS, CNRS (France)
Max Langer, Univ. Lyon, INSA Lyon, Univ. Claude Bernard Lyon 1, Univ. Jean Monnet Saint Etienne, CREATIS, CNRS (France)
ESRF - The European Synchrotron (France)
Alexandra Pacureanu, ESRF - The European Synchrotron (France)
Remy Gauthier, Univ. Lyon, Univ. Claude Bernard Lyon 1, IFSTTAR (France)
Helene Follet, Univ. Lyon, Univ. Claude Bernard Lyon 1, INSERM (France)
David Mitton, Univ. Lyon, Univ. Claude Bernard Lyon 1, IFSTTAR (France)
Cecile Olivier, Univ. Lyon, INSA Lyon, Univ. Claude Bernard Lyon 1, Univ. Jean Monnet Saint Etienne, CREATIS, CNRS (France)
ESRF - The European Sychrotron (France)
Peter Cloetens, ESRF - The European Synchrotron (France)
Francoise Peyrin, Univ. Lyon, INSA Lyon, Univ. Claude Bernard Lyon 1, Univ. Jean Monnet Saint Etienne, CREATIS, CNRS (France)
ESRF - The European Synchrotron (France)


Published in SPIE Proceedings Vol. 10391:
Developments in X-Ray Tomography XI
Bert Müller; Ge Wang, Editor(s)

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