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Parallel alignment of bacteria using near-field optical force array for cell sorting
Author(s): H. T. Zhao; Y. Zhang; L. K. Chin; P. H. Yap; K. Wang; W. Ser; A. Q. Liu
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Paper Abstract

This paper presents a near-field approach to align multiple rod-shaped bacteria based on the interference pattern in silicon nano-waveguide arrays. The bacteria in the optical field will be first trapped by the gradient force and then rotated by the scattering force to the equilibrium position. In the experiment, the Shigella bacteria is rotated 90 deg and aligned to horizontal direction in 9.4 s. Meanwhile, ~150 Shigella is trapped on the surface in 5 min and 86% is aligned with angle <; 5 deg. This method is a promising toolbox for the research of parallel single-cell biophysical characterization, cell-cell interaction, etc.

Paper Details

Date Published: 25 August 2017
PDF: 4 pages
Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 1034739 (25 August 2017); doi: 10.1117/12.2271939
Show Author Affiliations
H. T. Zhao, Nanyang Technological Univ. (Singapore)
Y. Zhang, Nanyang Technological Univ. (Singapore)
L. K. Chin, Nanyang Technological Univ. (Singapore)
P. H. Yap, Nanyang Technological Univ. (Singapore)
K. Wang, Taipei Medical Univ. (Taiwan)
W. Ser, Nanyang Technological Univ. (Singapore)
A. Q. Liu, Nanyang Technological Univ. (Singapore)


Published in SPIE Proceedings Vol. 10347:
Optical Trapping and Optical Micromanipulation XIV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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