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Proceedings Paper

Investigation on microfluidic particles manipulation by holographic 3D tracking strategies
Author(s): Teresa Cacace; Melania Paturzo; Pasquale Memmolo; Massimo Vassalli; Massimiliano Fraldi; Giuseppe Mensitieri; Pietro Ferraro
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Paper Abstract

We demonstrate a 3D holographic tracking method to investigate particles motion in a microfluidic channel while unperturbed while inducing their migration through microfluidic manipulation. Digital holography (DH) in microscopy is a full-field, label-free imaging technique able to provide quantitative phase-contrast. The employed 3D tracking method is articulated in steps. First, the displacements along the optical axis are assessed by numerical refocusing criteria. In particular, an automatic refocusing method to recover the particles axial position is implemented employing a contrast-based refocusing criterion. Then, the transverse position of the in-focus object is evaluated through quantitative phase map segmentation methods and centroid-based 2D tracking strategy. The introduction of DH is thus suggested as a powerful approach for control of particles and biological samples manipulation, as well as a possible aid to precise design and implementation of advanced lab-on-chip microfluidic devices.

Paper Details

Date Published: 26 June 2017
PDF: 6 pages
Proc. SPIE 10333, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III, 1033302 (26 June 2017); doi: 10.1117/12.2271829
Show Author Affiliations
Teresa Cacace, Istituto di Scienze Applicata e Sistemi Intelligenti-CNR (Italy)
Univ. of Campania “L. Vanvitelli” (Italy)
Melania Paturzo, Istituto di Scienze Applicata e Sistemi Intelligenti-CNR (Italy)
Pasquale Memmolo, Istituto di Scienze Applicata e Sistemi Intelligenti-CNR (Italy)
Massimo Vassalli, Consiglio Nazionale delle Ricerche (Italy)
Massimiliano Fraldi, Univ. degli Studi di Napoli Federico II (Italy)
Giuseppe Mensitieri, Univ. degli Studi di Napoli Federico II (Italy)
Pietro Ferraro, Istituto di Scienze Applicata e Sistemi Intelligenti-CNR (Italy)


Published in SPIE Proceedings Vol. 10333:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; Christoph K. Hitzenberger, Editor(s)

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