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Holographic 3D multi-spot two-photon excitation for fast optical stimulation in brain
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Paper Abstract

We report here a holographic high speed accessing microscope of sensory-driven synaptic activity across all inputs to single living neurons in the context of the intact cerebral cortex. This system is based on holographic multiple beam generation with spatial light modulator, we have demonstrated performance of the holographic excitation efficiency in several in vitro prototype system. 3D weighted iterative Fourier Transform method using the Ewald sphere in consideration of calculation speed has been adopted; multiple locations can be patterned in 3D with single hologram. Standard deviation of intensities of spots are still large due to the aberration of the system and/or hologram calculation, we successfully excited multiple locations of neurons in living mouse brain to monitor the calcium signals.

Paper Details

Date Published: 18 April 2017
PDF: 2 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025117 (18 April 2017); doi: 10.1117/12.2271607
Show Author Affiliations
Yu Takiguchi, Hamamatsu Photonics K.K. (Japan)
Massachusetts Institute of Technology (United States)
Haruyoshi Toyoda, Hamamatsu Photonics K.K. (Japan)


Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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