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Proceedings Paper

A localized surface plasmon resonance (LSPR) immunosensor for CRP detection using 4-chloro-1-naphtol (4-CN) precipitation
Author(s): Su-Ji Ha; Jin-Ho Park; Ju-Young Byun; Young-Deok Ahn; Min-Gon Kim
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Paper Abstract

In this study, C-reactive protein (CRP) was detected by monitoring of LSPR shift promoted by precipitation of 4-chloro-1-naphthol (4-CN). The precipitation occurred by horseradish peroxide (HRP) catalyst which is modified at CRP-detection antibody utilized in sandwich enzyme-linked immunosorbent assay (ELISA) on gold nano bipyramid (GNBP) substrate. Due to 4-CN precipitates which are located nearby the surface of GNBP, local refractive index (RI) and molecular density were greatly increased. This phenomenon eventually induced strong spectral red-shift of absorption band of GNBP. An excellent linear relationship (R2=0.9895) between the LSPR shift and CRP concentration was obtained in the range from 100 pg/mL to 100 ng/mL and limit of detection (LOD) was reached to 87 pg/mL.

Paper Details

Date Published: 12 July 2017
PDF: 5 pages
Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 103240E (12 July 2017); doi: 10.1117/12.2271397
Show Author Affiliations
Su-Ji Ha, Gwangju Institute of Science and Technology (Korea, Republic of)
Jin-Ho Park, Gwangju Institute of Science and Technology (Korea, Republic of)
Ju-Young Byun, Korea Research Institute of Bioscience & Biotechnology (Korea, Republic of)
Young-Deok Ahn, Gwangju Institute of Science and Technology (Korea, Republic of)
Min-Gon Kim, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 10324:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
Jaebum Choo; Seung-Han Park, Editor(s)

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