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Multimodal nonlinear imaging of arabidopsis thaliana root cell
Author(s): Bumjoon Jang; Sung-Ho Lee; Sooah Woo; Jong-Hyun Park; Myeong Min Lee; Seung-Han Park
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Paper Abstract

Nonlinear optical microscopy has enabled the possibility to explore inside the living organisms. It utilizes ultrashort laser pulse with long wavelength (greater than 800nm). Ultrashort pulse produces high peak power to induce nonlinear optical phenomenon such as two-photon excitation fluorescence (TPEF) and harmonic generations in the medium while maintaining relatively low average energy pre area. In plant developmental biology, confocal microscopy is widely used in plant cell imaging after the development of biological fluorescence labels in mid-1990s. However, fluorescence labeling itself affects the sample and the sample deviates from intact condition especially when labelling the entire cell. In this work, we report the dynamic images of Arabidopsis thaliana root cells. This demonstrates the multimodal nonlinear optical microscopy is an effective tool for long-term plant cell imaging.

Paper Details

Date Published: 12 July 2017
PDF: 5 pages
Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 103240C (12 July 2017); doi: 10.1117/12.2270697
Show Author Affiliations
Bumjoon Jang, Yonsei Univ. (Korea, Republic of)
Sung-Ho Lee, Yonsei Univ. (Korea, Republic of)
Sooah Woo, Yonsei Univ. (Korea, Republic of)
Jong-Hyun Park, Yonsei Univ. (Korea, Republic of)
Myeong Min Lee, Yonsei Univ. (Korea, Republic of)
Seung-Han Park, Yonsei Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 10324:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
Jaebum Choo; Seung-Han Park, Editor(s)

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