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Proceedings Paper

Investigation of light scattering characteristics of individual leukocytes using three-dimensional refractive index maps
Author(s): Kung-Bin Sung; Yang-Hsien Lin; Fong-jheng Lin; Chao-Mao Hsieh; Shang-Ju Wu
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Paper Abstract

Three-dimensional (3D) refractive-index (RI) microscopy is an emerging technique suitable for live-cell imaging due to its label-free and fast 3D imaging capabilities. We have developed a common-path system to acquire 3D RI microscopic images of cells with excellent speed and stability. After obtaining 3D RI distributions of individual leukocytes, we used a 3D finite-difference time-domain tool to study light scattering properties. Backscattering spectra of lymphocytes, monocytes and neutrophils are different from each other. Backscattering spectra of lymphocytes matched well with those of homogeneous spheres as predicted by Mie theory while backscattering spectra of neutrophils are significantly more intense than those of the other two types. This suggests the possibility of classifying the three types of leukocytes based on backscattering.

Paper Details

Date Published: 18 April 2017
PDF: 2 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025109 (18 April 2017); doi: 10.1117/12.2270427
Show Author Affiliations
Kung-Bin Sung, National Taiwan Univ. (Taiwan)
Yang-Hsien Lin, National Taiwan Univ. (Taiwan)
Fong-jheng Lin, National Taiwan Univ. (Taiwan)
Chao-Mao Hsieh, National Taiwan Univ. (Taiwan)
Shang-Ju Wu, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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