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Proceedings Paper

Functionalized gold nanostars for label-free detection of PKA phosphorylation using surface-enhanced Raman spectroscopy
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Paper Abstract

Protein phosphorylation controls fundamental biological processes. Dysregulation of protein kinase is associated with a series of human diseases including cancer. Protein kinase A (PKA) activity has been reported to serve as a potential prognostic marker for cancer. To this end, we developed a non-radioactive, rapid, cheap and robust scheme based on surface-enhanced Raman spectroscopy (SERS) for label-free detection of PKA phosphorylation using gold nanostars (AuNS) functionalized with BSA-kemptide. While bovine serum albumin (BSA) proteins stabilized the AuNS, kemptide, which is a high affinity substrate peptide specific for PKA, were phosphorylated in vitro to generate Raman signals that were identified by performing principal component analysis (PCA) on the acquired SERS spectra.

Paper Details

Date Published: 29 April 2017
PDF: 4 pages
Proc. SPIE 10340, International Conference on Biophotonics V, 103400W (29 April 2017); doi: 10.1117/12.2269722
Show Author Affiliations
Shuai He, National Univ. of Singapore (Singapore)
James Chen Yong Kah, National Univ. of Singapore (Singapore)

Published in SPIE Proceedings Vol. 10340:
International Conference on Biophotonics V
David D. Sampson; Dennis L. Matthews; Jürgen Popp; Halina Rubinsztein-Dunlop; Brian C. Wilson, Editor(s)

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