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Proceedings Paper

Intrinsic fluorescence spectra of tryptophan, tyrosine and phenyloalanine
Author(s): Hui Yang; Xue Xiao; Xuesong Zhao; Yan Wu
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Paper Abstract

This paper presents the intrinsic fluorescence spectra characteristics of tryptophan, tyrosine and phenyloalanine measured with 3D fluorescence spectrophotometer. Two strong fluorescence peaks of tryptophan locate at λex/λem=230/348nm and 280/348nm, three strong fluorescence peaks of tyrosine locate at λex/λem=202/304nm, λex/λem=220/304nm and 275/304nm, and two fluorescence peaks of phenyloalanine locate at λex/λem=210/280nm and λex/λem=260/280nm were found. The linear relationship of fluorescence intensity to solution concentration were also observed in condition of low solution concentration. The influence of pH of solution to the fluorescence intensity was also measured and discussed.

Paper Details

Date Published: 8 March 2017
PDF: 8 pages
Proc. SPIE 10255, Selected Papers of the Chinese Society for Optical Engineering Conferences held October and November 2016, 102554M (8 March 2017); doi: 10.1117/12.2268397
Show Author Affiliations
Hui Yang, New Star Research Institute of Applied Technology (China)
Xue Xiao, Anhui Institute of Optics and Fine Mechanics (China)
Xuesong Zhao, Anhui Institute of Optics and Fine Mechanics (China)
Yan Wu, New Star Research Institute of Applied Technology (China)


Published in SPIE Proceedings Vol. 10255:
Selected Papers of the Chinese Society for Optical Engineering Conferences held October and November 2016
Yueguang Lv; Jialing Le; Hesheng Chen; Jianyu Wang; Jianda Shao, Editor(s)

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