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Optimal design of dark field illumination optical system for the bacterial colony imaging and selection device
Author(s): Jihong Zheng; Kangni Wang; Lu Liu; Xiaopeng Wei; Daoping Li; Rongfu Zhang; Songlin Zhuang
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Paper Abstract

Dark field illumination in high throughput colony selection device is reported in this paper. It improves the contrast of image in the premise of uniform illumination. Three important factors including angular distribution of light radiation, number of LED circle array and distance between the target and the LED circle source were analyzed, which affect illumination uniformity of dark field illumination. The simulation result shows that the illumination uniformity is 93.16% when the angular distribution of light radiation is 75°, the number of LED circle array is 3, and the distance between the target and the LED circle source is 61mm. In addition, Experimental system was set up in order to test designed dark field illumination, which matches with the project requirement and simulated results very well.

Paper Details

Date Published: 5 May 2017
PDF: 11 pages
Proc. SPIE 10209, Image Sensing Technologies: Materials, Devices, Systems, and Applications IV, 102090Z (5 May 2017); doi: 10.1117/12.2267987
Show Author Affiliations
Jihong Zheng, Univ. of Shanghai for Science and Technology (China)
Kangni Wang, Univ. of Shanghai for Science and Technology (China)
Lu Liu, Univ. of Shanghai for Science and Technology (China)
Xiaopeng Wei, Univ. of Shanghai for Science and Technology (China)
Daoping Li, Univ. of Shanghai for Science and Technology (China)
Rongfu Zhang, Univ. of Shanghai for Science and Technology (China)
Songlin Zhuang, Univ. of Shanghai for Science and Technology (China)
Key Lab. of Modern Optical System, University of Shanghai for Science and Technology (China)


Published in SPIE Proceedings Vol. 10209:
Image Sensing Technologies: Materials, Devices, Systems, and Applications IV
Nibir K. Dhar; Achyut K. Dutta, Editor(s)

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