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Proceedings Paper

Different reaction of the core histones H2A and H2B to red laser irradiation
Author(s): G. E. Brill; A. V. Egorova; I. O. Bugaeva; D. E. Postnov; O. V. Ushakova
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Paper Abstract

Analysis of the influence of red laser irradiation on the processes of self-assembly of the core histones H2A and H2B was performed using a wedge dehydration method. Image-analysis of facies included their qualitative characteristics and calculation of quantitative parameters with subsequent statistical processing. It was established that linearly polarized red laser light (λ - 660 nm, 1 J/cm2) significantly modified the process of self-assembly of core histone H2B, whereas the structure of the facies of H2A histone changed to a lesser extent. Histones were used in the form of aqueous salt solutions. The effect of red light seems to result from the formation of singlet oxygen by direct laser excitation of molecular oxygen.

Paper Details

Date Published: 24 March 2017
PDF: 5 pages
Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360G (24 March 2017); doi: 10.1117/12.2267949
Show Author Affiliations
G. E. Brill, Saratov State Medical Univ. (Russian Federation)
A. V. Egorova, Saratov State Medical Univ. (Russian Federation)
I. O. Bugaeva, Saratov State Medical Univ. (Russian Federation)
D. E. Postnov, Saratov State Univ. (Russian Federation)
O. V. Ushakova, Yuri Gagarin State Technical Univ. of Saratov (Russian Federation)


Published in SPIE Proceedings Vol. 10336:
Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII
Valery V. Tuchin; Elina A. Genina; Dmitry E. Postnov; Vladimir L. Derbov, Editor(s)

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