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Proceedings Paper

Fluorescent angiography of chicken embryo and photobleaching velocimetry
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Paper Abstract

Fluorescent angiography approach in application to a living chicken embryo is discussed. It provides precise vessel wall detection and demonstrates usefulness for real time monitoring of vasoconstriction and vasodilatation related to self regulation of vascular network as well as to response to external factors. On the other hand, high stability of fluorescence and long period of dye elimination makes variations of fluorescent intensity practically independent from fast variations of blood flow rate. Therefore, we proposed the improvement of fluorescent angiography technique by introduction of photobleaching fluorescent velocimetry approach. We have developed the imaging system for intravital microscopic photobleaching velocimetry and tested it by using a glass capillary tube as a model of blood vessel. We demonstrated high potential of the technique for instant flow velocity distribution profile measurement with high spatial and temporal resolution up to 2 μm and 60 ms, respectively.

Paper Details

Date Published: 24 March 2017
PDF: 9 pages
Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360V (24 March 2017); doi: 10.1117/12.2267948
Show Author Affiliations
Anton A. Namykin, National Research Saratov State Univ. (Russian Federation)
Elena S. Stiukhina, National Research Saratov State Univ. (Russian Federation)
Ivan V. Fedosov, National Research Saratov State Univ. (Russian Federation)
Dmitry E. Postnov, National Research Saratov State Univ. (Russian Federation)
Valery V. Tuchin, National Research Saratov State Univ. (Russian Federation)
Institute of Precision Mechanics and Control (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 10336:
Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII
Valery V. Tuchin; Elina A. Genina; Dmitry E. Postnov; Vladimir L. Derbov, Editor(s)

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