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Proceedings Paper

Optical methods for the evaluation of film-forming ability of UPEC on the surface of medical devices
Author(s): Basim M. Al-Bayati; Olga V. Nechaeva; Vladimir Yu. Ulyanov; Elena V. Glinskaya; Dmitry A. Zayarsky; Elena I. Tikhomirova; Malika M. Vakaraeva; Natalia V. Bespalova; Anna V. Egorova
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Paper Abstract

The current study was designed to investigate the dynamics of microbial biofilm-formation in standard and clinical strains of uropathogenic Escherichia coli (UPEC), using spectrophotometric and electron microscopic methods. For achieving this goal, three E. coli strains were used (one standard and two clinical). These isolates differ in the existence of fimH virulence gene. The process involved the using of electron microscopy and immunological microtitration plates under in vitro conditions for 96 hours. We found that standard and clinical strains of E. coli were capable of forming a microbial biofilm in vitro, with a higher intensity related to the clinical strain E. coli 245. The using of scanning electron microscopy confirmed the obtained results. The combined use of different optical methods gives a complete characterization of film-forming ability of pathogenic microorganisms.

Paper Details

Date Published: 24 March 2017
PDF: 4 pages
Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360E (24 March 2017); doi: 10.1117/12.2267860
Show Author Affiliations
Basim M. Al-Bayati, National Research Saratov State Univ. (Russian Federation)
Olga V. Nechaeva, Saratov State Medical Univ. (Russian Federation)
Vladimir Yu. Ulyanov, Saratov Research Institute of Traumatology and Orthopedics (Russian Federation)
Elena V. Glinskaya, National Research Saratov State Univ. (Russian Federation)
Dmitry A. Zayarsky, Saratov State Technical Univ. (Russian Federation)
Elena I. Tikhomirova, Saratov State Technical Univ. (Russian Federation)
Malika M. Vakaraeva, Chechen State Univ. (Russian Federation)
Natalia V. Bespalova, Saratov State Technical Univ. (Russian Federation)
Anna V. Egorova, Saratov State Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 10336:
Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII
Valery V. Tuchin; Elina A. Genina; Dmitry E. Postnov; Vladimir L. Derbov, Editor(s)

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