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Proceedings Paper

Supercritical angle fluorescence as a tool to study the interaction between lipid bilayer and peptides
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Paper Abstract

The understanding of processes occurring at the interface between two media are of prior importance in various fields of research, from material sciences to biology. A custom-made microscope objective based on the supercritical angle technique was developed in our group, allowing to probe these interfacial events by carrying out surface-sensitive and low invasive spectroscopy of aqueous samples. A biological example of particular interest is the comprehension of neurodegenerative diseases which seem caused by the interaction of specific peptides with the membrane of the neurons. Taking advantage of our optical setup, we used supercritical angle fluorescence spectroscopy to specifically monitor the interaction between a supported lipid bilayer (SLB) and the Amyloid β peptide, notably responsible of the Alzheimer disease. Different forms of the peptide (40 and 42 amino acids composition) were tested and the interfacial fluorescence measured to get information about the lipid integrity and mobility. The adsorption of the peptide was also characterized in terms of kinetic and affinity.

Paper Details

Date Published: 9 June 2017
PDF: 7 pages
Proc. SPIE 10231, Optical Sensors 2017, 102312E (9 June 2017); doi: 10.1117/12.2267140
Show Author Affiliations
Valentin Dubois, Univ. of Zürich (Switzerland)
Diana Serrano, Univ. of Zürich (Switzerland)
Stefan Seeger, Univ. of Zürich (Switzerland)


Published in SPIE Proceedings Vol. 10231:
Optical Sensors 2017
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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