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Proceedings Paper

Computational method for multi-modal microscopy based on transport of intensity equation
Author(s): Jiaji Li; Qian Chen; Jiasong Sun; Jialin Zhang; Chao Zuo
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Paper Abstract

In this paper, we develop the requisite theory to describe a hybrid virtual-physical multi-modal imaging system which yields quantitative phase, Zernike phase contrast, differential interference contrast (DIC), and light field moment imaging simultaneously based on transport of intensity equation(TIE). We then give the experimental demonstration of these ideas by time-lapse imaging of live HeLa cell mitosis. Experimental results verify that a tunable lens based TIE system, combined with the appropriate post-processing algorithm, can achieve a variety of promising imaging modalities in parallel with the quantitative phase images for the dynamic study of cellular processes.

Paper Details

Date Published: 10 February 2017
PDF: 4 pages
Proc. SPIE 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016), 102501L (10 February 2017); doi: 10.1117/12.2266905
Show Author Affiliations
Jiaji Li, Nanjing Univ. of Science and Technology (China)
Qian Chen, Nanjing Univ. of Science and Technology (China)
Jiasong Sun, Nanjing Univ. of Science and Technology (China)
Jialin Zhang, Nanjing Univ. of Science and Technology (China)
Chao Zuo, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10250:
International Conference on Optical and Photonics Engineering (icOPEN 2016)
Anand Krishna Asundi; Xiyan Huang; Yi Xie, Editor(s)

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