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Proceedings Paper

The design of rapid MicroRNA detection system
Author(s): Yanfei Wang; Dongsheng Yu; Haiyan Chen; Zhanying Zhang; Weikai Fang; Zeyuan Lu; Yanlei Li; Yufeng Ji; Yifu Guan; Chidong Xu; Haihe Jiang
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Paper Abstract

In order to detect miRNA quickly, we designed a new portable device for the rapid detection of miRNA, using Opto-electronic detection technology, marking miRNA and isothermal rolling circle amplification and detecting markers which excite fluorescence intensity, the recognition system of characteristic fluorescence analysis was established. By changing the excitation light intensity, miRNA reagent concentration and other parameters, we arrive at the conclusion that there is the linear relationship (R2=0.9947) between miRNA concentration and fluorescence intensity when the miRNA concentration range the instrument can measure is in the range of 0.01-0.1mol and the lowest values measured by the instrument in theory is 7 copies.

Paper Details

Date Published: 5 January 2017
PDF: 8 pages
Proc. SPIE 10244, International Conference on Optoelectronics and Microelectronics Technology and Application, 102440W (5 January 2017); doi: 10.1117/12.2264423
Show Author Affiliations
Yanfei Wang, Ctr. of Medical Physics and Technology (China)
Univ. of Science and Technology China (China)
Dongsheng Yu, Ctr. of Medical Physics and Technology (China)
Haiyan Chen, Ctr. of Medical Physics and Technology (China)
Zhanying Zhang, Ctr. of Medical Physics and Technology (China)
Weikai Fang, Ctr. of Medical Physics and Technology (China)
Zeyuan Lu, Ctr. of Medical Physics and Technology (China)
Yanlei Li, Ctr. of Medical Physics and Technology (China)
Yufeng Ji, Ctr. of Medical Physics and Technology (China)
Yifu Guan, Ctr. of Medical Physics and Technology (China)
China Medical Univ. (China)
Chidong Xu, Ctr. of Medical Physics and Technology (China)
Haihe Jiang, Ctr. of Medical Physics and Technology (China)


Published in SPIE Proceedings Vol. 10244:
International Conference on Optoelectronics and Microelectronics Technology and Application
Shaohua Yu; Jose Capmany; Yi Luo; Yikai Su; Songlin Zhuang; Yue Hao; Akihiko Yoshikawa; Chongjin Xie; Yoshiaki Nakano, Editor(s)

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