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Proceedings Paper

High sensitive and selective Escherichia coli detection using immobilized optical fiber microprobe
Author(s): Yanpeng Li; Qizhen Sun; Yiyang Luo; Yue Li; Andong Gong; Haibin Zhang; Deming Liu
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Paper Abstract

We proposed and demonstrated a stable, label-free bacteriophage-based sensor of Escherichia coli using microfiber probe. T4 Bacteriophage was covalently immobilized on microfiber surface and E.coli concentration was investigated using the high accurate spectral interference mechanism. By immersing microfiber sensor into different concentration E.coli solution, the relationship between resonant wavelength shift and E.coli concentration was analyzed in the range of 103-107cfu/ml. The proposed method is capable of reliable detection of E.coli concentration as low as 103cfu/ml with a fast response time about 10minutes, which makes the real-time detection of E.coli move on a giant step. Additionally, the sensor has great potential to be applied in the fields like environment monitoring and food safety.

Paper Details

Date Published: 23 April 2017
PDF: 4 pages
Proc. SPIE 10323, 25th International Conference on Optical Fiber Sensors, 103232P (23 April 2017); doi: 10.1117/12.2263456
Show Author Affiliations
Yanpeng Li, Huazhong Univ. of Science and Technology (China)
Qizhen Sun, Huazhong Univ. of Science and Technology (China)
Yiyang Luo, Huazhong Univ. of Science and Technology (China)
Yue Li, Huazhong Univ. of Science and Technology (China)
Andong Gong, Xinyang Normal Univ. (China)
Haibin Zhang, Xinyang Normal Univ. (China)
Deming Liu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10323:
25th International Conference on Optical Fiber Sensors
Youngjoo Chung; Wei Jin; Byoungho Lee; John Canning; Kentaro Nakamura; Libo Yuan, Editor(s)

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