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Proceedings Paper

Determination of polarizability and surface concentration of biomolecules using surface plasmon resonance experiment
Author(s): Yuri M. Shirshov; Vladimir I. Chegel; Yuri V. Subbota; E. P. Matsas; A. E. Rachcov; T. A. Sergeeva
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Paper Abstract

Both surface molecules concentration Ns and polarizability Am can be determined from the surface plasmon experiment instead of effective layer thickness, which is usually determined. The new method of quantitative comparison of experimental and calculated surface plasmon resonance (SPR) curves is proposed. Mathematical minimization of curves difference is used in this case. It is shown, that it is possible to determine both Am and Nm from one measurement for accurate experiment and high quality optical system.

Paper Details

Date Published: 3 November 1995
PDF: 6 pages
Proc. SPIE 2648, International Conference on Optical Diagnostics of Materials and Devices for Opto-, Micro-, and Quantum Electronics, (3 November 1995); doi: 10.1117/12.226151
Show Author Affiliations
Yuri M. Shirshov, Institute of Semiconductor Physics (Ukraine)
Vladimir I. Chegel, Institute of Semiconductor Physics (Ukraine)
Yuri V. Subbota, Institute of Semiconductor Physics (Ukraine)
E. P. Matsas, Institute of Semiconductor Physics (Ukraine)
A. E. Rachcov, Institute of Molecular Biology and Genetics (Ukraine)
T. A. Sergeeva, Institute of Molecular Biology and Genetics (Ukraine)


Published in SPIE Proceedings Vol. 2648:
International Conference on Optical Diagnostics of Materials and Devices for Opto-, Micro-, and Quantum Electronics
Sergey V. Svechnikov; Mikhail Ya. Valakh, Editor(s)

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