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Proceedings Paper

Application of fluorescence spectroscopy and imaging in the detection of a photosensitizer in photodynamic therapy
Author(s): Lixin Zang; Huimin Zhao; Zhiguo Zhang; Wenwu Cao
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Paper Abstract

Photodynamic therapy (PDT) is currently an advanced optical technology in medical applications. However, the application of PDT is limited by the detection of photosensitizers. This work focuses on the application of fluorescence spectroscopy and imaging in the detection of an effective photosenzitizer, hematoporphyrin monomethyl ether (HMME). Optical properties of HMME were measured and analyzed based on its absorption and fluorescence spectra. The production mechanism of its fluorescence emission was analyzed. The detection device for HMME based on fluorescence spectroscopy was designed. Ratiometric method was applied to eliminate the influence of intensity change of excitation sources, fluctuates of excitation sources and photo detectors, and background emissions. The detection limit of this device is 6 μg/L, and it was successfully applied to the diagnosis of the metabolism of HMME in the esophageal cancer cells. To overcome the limitation of the point measurement using fluorescence spectroscopy, a two-dimensional (2D) fluorescence imaging system was established. The algorithm of the 2D fluorescence imaging system is deduced according to the fluorescence ratiometric method using bandpass filters. The method of multiple pixel point addition (MPPA) was used to eliminate fluctuates of signals. Using the method of MPPA, SNR was improved by about 30 times. The detection limit of this imaging system is 1.9 μg/L. Our systems can be used in the detection of porphyrins to improve the PDT effect.

Paper Details

Date Published: 28 February 2017
PDF: 7 pages
Proc. SPIE 10256, Second International Conference on Photonics and Optical Engineering, 102565A (28 February 2017); doi: 10.1117/12.2261076
Show Author Affiliations
Lixin Zang, Harbin Institute of Technology (China)
Huimin Zhao, Harbin Institute of Technology (China)
Zhiguo Zhang, Harbin Institute of Technology (China)
Wenwu Cao, Harbin Institute of Technology (China)
The Pennsylvania State Univ. (United States)


Published in SPIE Proceedings Vol. 10256:
Second International Conference on Photonics and Optical Engineering
Chunmin Zhang; Anand Asundi, Editor(s)

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