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Proceedings Paper

Molecular counting of membrane receptor subunits with single-molecule localization microscopy
Author(s): Carmen Krüger; Franziska Fricke; Christos Karathanasis; Marina S. Dietz; Sebastian Malkusch; Gerhard Hummer; Mike Heilemann
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Paper Abstract

We report on quantitative single-molecule localization microscopy, a method that next to super-resolved images of cellular structures provides information on protein copy numbers in protein clusters. This approach is based on the analysis of blinking cycles of single fluorophores, and on a model-free description of the distribution of the number of blinking events. We describe the experimental and analytical procedures, present cellular data of plasma membrane proteins and discuss the applicability of this method.

Paper Details

Date Published: 21 February 2017
PDF: 11 pages
Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710K (21 February 2017); doi: 10.1117/12.2256638
Show Author Affiliations
Carmen Krüger, Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)
Franziska Fricke, Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)
Christos Karathanasis, Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)
Marina S. Dietz, Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)
Sebastian Malkusch, Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)
Gerhard Hummer, Max Planck Institute of Biophysics (Germany)
Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)
Mike Heilemann, Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)


Published in SPIE Proceedings Vol. 10071:
Single Molecule Spectroscopy and Superresolution Imaging X
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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