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Proceedings Paper

16-Ch time-resolved single-molecule spectroscopy using line excitation
Author(s): Antonino Ingargiola; Pietro Peronio; Eitan Lerner; Angelo Gulinatti; Ivan Rech; Massimo Ghioni; Shimon Weiss; Xavier Michalet
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Paper Abstract

Single-molecule spectroscopy on freely-diffusing molecules allows detecting conformational changes of biomolecules without perturbation from surface immobilization. Resolving fluorescence lifetimes increases the sensitivity in detecting conformational changes and overcomes artifacts common in intensity-based measurements. Common to all freely-diffusing techniques, however, are the long acquisition times. We report a time-resolved multispot system employing a 16-channel SPAD array and TCSPC electronics, which overcomes the throughput issue. Excitation is obtained by shaping a 532 nm pulsed laser into a line, matching the linear SPAD array geometry. We show that the line-excitation is a robust and cost-effective approach to implement multispot systems based on linear detector arrays.

Paper Details

Date Published: 2 March 2017
PDF: 12 pages
Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710Q (2 March 2017); doi: 10.1117/12.2256367
Show Author Affiliations
Antonino Ingargiola, Univ. of California, Los Angeles (United States)
Pietro Peronio, Politecnico di Milano (Italy)
Eitan Lerner, Univ. of California Los Angeles (United States)
Angelo Gulinatti, Politecnico di Milano (Italy)
Ivan Rech, Politecnico di Milano (Italy)
Massimo Ghioni, Politecnico di Milano (Italy)
Shimon Weiss, Univ. of California, Los Angeles (United States)
Xavier Michalet, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 10071:
Single Molecule Spectroscopy and Superresolution Imaging X
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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