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Proceedings Paper

High-sensitivity chemical imaging for biomedicine by SRS microscopy (Conference Presentation)
Author(s): Wei Min

Paper Abstract

Innovations in spectroscopy principles and microscopy technology have significantly impacted modern biology and medicine. While most of the contemporary bio-imaging modalities harness electronic transition, nuclear spin or radioactivity, vibrational spectroscopy has not been widely used yet. Here we will discuss an emerging chemical imaging platform, stimulated Raman scattering (SRS) microscopy, which can enhance the otherwise feeble spontaneous Raman eight orders of magnitude by virtue of stimulated emission. When coupled with stable isotopes (e.g., deuterium and 13C) or bioorthogonal chemical moieties (e.g., alkynes), SRS microscopy is well suited for probing in vivo metabolic dynamics of small bio-molecules which cannot be labeled by bulky fluorophores. Physical principle of the underlying optical spectroscopy and exciting biomedical applications such as imaging lipid metabolism, protein synthesis, DNA replication, protein degradation, RNA synthesis, glucose uptake, drug trafficking and tumor metabolism will be presented.

Paper Details

Date Published: 24 April 2017
PDF: 1 pages
Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 1007602 (24 April 2017); doi: 10.1117/12.2256239
Show Author Affiliations
Wei Min, Columbia Univ. (United States)


Published in SPIE Proceedings Vol. 10076:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II
Kevin K. Tsia; Keisuke Goda, Editor(s)

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