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Proceedings Paper

Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles
Author(s): Jun Ando; Takumasa Sekiya; Den Ka; Hiroyuki Yamakoshi; Kosuke Dodo; Mikiko Sodeoka; Satoshi Kawata; Katsumasa Fujita
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Paper Abstract

We propose the combination of alkyne-tag and surface-enhanced Raman scattering (SERS) spectroscopy to perform highly-sensitive and selective drug imaging in live cells. Gold nanoparticles are introduced in lysosomes through endocytosis as SERS agents, and the alkyne-tagged drugs are subsequently administered in cells. Raman microscopic observation reveals the arrival of drug in lysosome through enhanced Raman signal of alkyne. Since the peak of alkyne appears in Raman-silent region of biomolecules, selective detection of drugs is possible without background signal of endogenous molecules. From endocytosed gold nanoparticles in living HeLa cells, we observed distinct Raman signal from alkyne-tagged inhibitor of lysosomal enzyme.

Paper Details

Date Published: 9 February 2017
PDF: 6 pages
Proc. SPIE 10046, Visualizing and Quantifying Drug Distribution in Tissue, 1004606 (9 February 2017); doi: 10.1117/12.2256126
Show Author Affiliations
Jun Ando, Osaka Univ. (Japan)
RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Takumasa Sekiya, Osaka Univ. (Japan)
Den Ka, Osaka Univ. (Japan)
Hiroyuki Yamakoshi, RIKEN (Japan)
Kosuke Dodo, RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Mikiko Sodeoka, RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Japan Agency for Medical Research and Development (Japan)


Published in SPIE Proceedings Vol. 10046:
Visualizing and Quantifying Drug Distribution in Tissue
Kin Foong Chan; Conor L. Evans, Editor(s)

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