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Proceedings Paper

1H magnetic resonance spectroscopy metabolite profiles of neonatal rat hippocampus and brainstem regions following early postnatal exposure to intermittent hypoxia
Author(s): Robert A. Darnall; Xi Chen; Krishnamurthy V. Nemani; Chrystelle M. Sirieix; Barjor Gimi
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Paper Abstract

Most premature infants born at less than 30 weeks gestation are exposed to periods of mild intermittent hypoxia (IH) associated with apnea of prematurity and periodic breathing. In adults, IH associated with sleep apnea causes neurochemical and structural alterations in the brain. However, it is unknown whether IH in the premature infant leads to neurodevelopmental impairment. Quantification of biochemical markers that can precisely identify infants at risk of adverse neurodevelopmental outcome is essential. In vivo 1H magnetic resonance spectroscopy (1H MRS) facilitates the quantification of metabolites from distinct regions of the developing brain. We report the changes in metabolite profiles in the brainstem and hippocampal regions of developing rat brains, resulting from exposure to IH. Rat pups were chosen for study because there is rapid postnatal hippocampal development that occurs during the first 4 weeks in the developing rat brain, which corresponds to the first 2–3 postnatal years of development in humans. The brainstem was examined because of our interest in respiratory control disorders in the newborn and because of brainstem gliosis described in infants who succumb to Sudden Infant Death Syndrome (SIDS). Metabolite profiles were compared between hypoxia treated rat pups (n = 9) and normoxic controls (n = 6). Metabolite profiles were acquired using the Point-RESolved spectroscopy (PRESS) MRS sequence and were quantified using the TARQUIN software. There was a significant difference in the concentrations of creatine (p = 0.031), total creatine (creatine + phosphocreatine) (p = 0.028), and total choline (p = 0.001) in the brainstem, and glycine (p = 0.031) in the hippocampal region. The changes are consistent with altered cellular bioenergetics and metabolism associated with hypoxic insult.

Paper Details

Date Published: 13 March 2017
PDF: 5 pages
Proc. SPIE 10137, Medical Imaging 2017: Biomedical Applications in Molecular, Structural, and Functional Imaging, 101371Y (13 March 2017); doi: 10.1117/12.2255956
Show Author Affiliations
Robert A. Darnall, Geisel School of Medicine at Dartmouth (United States)
Xi Chen, Geisel School of Medicine at Dartmouth (United States)
Krishnamurthy V. Nemani, Geisel School of Medicine at Dartmouth (United States)
Chrystelle M. Sirieix, Geisel School of Medicine at Dartmouth (United States)
Barjor Gimi, Geisel School of Medicine at Dartmouth (United States)


Published in SPIE Proceedings Vol. 10137:
Medical Imaging 2017: Biomedical Applications in Molecular, Structural, and Functional Imaging
Andrzej Krol; Barjor Gimi, Editor(s)

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