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Proceedings Paper

Novel fluorescence nanobubbles for contrast-enhanced ultrasound imaging in rabbit VX2 hepatocellular carcinoma model
Author(s): Houqiang Yu; Wei Wang; Xiaoling He; Qibing Zhou; Mingyue Ding
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Paper Abstract

Ultrasound contrast agents (UCAs) such as SonoVue or Optison have been used widely in clinic for contrast-enhanced vascular imaging. However, microbubbles UCAs display limitations in tumor-targeted imaging due to the large sizes, nanoscaled UCAs has consequently attracted increasing attentions. In this work, we synthesized nanobubbles (NBs) by ultrasonic cavitation method, then a fluorescent marker of Alexa Fluor 680 was conjugated to the shell in order to observe the localization of NBs in tumor tissue. Measurement of fundamental characteristics showed that the NBs had homogeneous distribution of mean diameter of 267.9 ± 19.2 nm and polydispersity index of 0.410 ± 0.056. To assess in vivo tumor-selectivity of NBs, we established the rabbits VX2 hepatocellular carcinoma model though surgical implantation method. After the rabbits were intravenous administered of NBs, contrast-enhanced sonograms was observed in the surrounding of VX2 tumor, which showed there are rich capillaries in the tumor periphery. We additionally investigated the toxic of the NBs by hematoxylin-eosin staining. The results indicated that the NBs is a biocompatible non-toxic lipid system. Furthermore, the VX2 tumors and major organs were analyzed using ex vivo fluorescence imaging to confirm the targeted selectivity of NBs, and the results verified that the NBs were capable of targeting VX2 tumor. Confocal laser scanning microscopy examination showed that the NBs can traverse the VX2 tumor capillaries and target to the hepatocellular carcinoma tumor cells. All these results suggested that the newly prepared NBs have a potential application in molecular imaging and tumor-targeting therapy.

Paper Details

Date Published: 13 March 2017
PDF: 9 pages
Proc. SPIE 10139, Medical Imaging 2017: Ultrasonic Imaging and Tomography, 101391M (13 March 2017); doi: 10.1117/12.2255624
Show Author Affiliations
Houqiang Yu, Huazhong Univ. of Science and Technology (China)
Wei Wang, Huazhong Univ. of Science and Technology (China)
Xiaoling He, Hospital of China Univ. of Geosciences (China)
Qibing Zhou, Huazhong Univ. of Science and Technology (China)
Mingyue Ding, Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 10139:
Medical Imaging 2017: Ultrasonic Imaging and Tomography
Neb Duric; Brecht Heyde, Editor(s)

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