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Proceedings Paper

Detection of cortical optical changes during seizure activity using optical coherence tomography (Conference Presentation)
Author(s): Danielle Ornelas; Md. Hasan; Oscar Gonzalez; Giri Krishnan; Jenny I. Szu; Timothy Myers; Koji Hirota; Maxim Bazhenov; Devin K. Binder; Boris H. Park
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Paper Abstract

Electrophysiology has remained the gold standard of neural activity detection but its resolution and high susceptibility to noise and motion artifact limit its efficiency. Imaging techniques, including fMRI, intrinsic optical imaging, and diffuse optical imaging, have been used to detect neural activity, but rely on indirect measurements such as changes in blood flow. Fluorescence-based techniques, including genetically encoded indicators, are powerful techniques, but require introduction of an exogenous fluorophore. A more direct optical imaging technique is optical coherence tomography (OCT), a label-free, high resolution, and minimally invasive imaging technique that can produce depth-resolved cross-sectional and 3D images. In this study, we sought to examine non-vascular depth-dependent optical changes directly related to neural activity. We used an OCT system centered at 1310 nm to search for changes in an ex vivo brain slice preparation and an in vivo model during 4-AP induced seizure onset and propagation with respect to electrical recording. By utilizing Doppler OCT and the depth-dependency of the attenuation coefficient, we demonstrate the ability to locate and remove the optical effects of vasculature within the upper regions of the cortex from in vivo attenuation calculations. The results of this study show a non-vascular decrease in intensity and attenuation in ex vivo and in vivo seizure models, respectively. Regions exhibiting decreased optical changes show significant temporal correlation to regions of increased electrical activity during seizure. This study allows for a thorough and biologically relevant analysis of the optical signature of seizure activity both ex vivo and in vivo using OCT.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, 100530O (19 April 2017); doi: 10.1117/12.2254947
Show Author Affiliations
Danielle Ornelas, Univ. of California, Riverside (United States)
Md. Hasan, Univ. of California, Riverside (United States)
Oscar Gonzalez, Univ. of California, San Diego (United States)
Giri Krishnan, Univ. of California, San Diego (United States)
Jenny I. Szu, Univ. of California, Riverside (United States)
Timothy Myers, Univ. of California, Riverside (United States)
Koji Hirota, Univ. of California, Riverside (United States)
Maxim Bazhenov, Univ. of California, San Diego (United States)
Devin K. Binder, Univ. of California, Riverside (United States)
Boris H. Park, Univ. of California, Riverside (United States)


Published in SPIE Proceedings Vol. 10053:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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