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Proceedings Paper

In vivo multiphoton imaging and quantification of cytoplasmic and nuclear metabolism in the hepatobiliary system
Author(s): Chih-Ju Lin; Ning Kang; Jian-Ye Lee; Hung-Ming Lin; Hsuan-Shu Lee; Chen-Yuan Dong
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Paper Abstract

Liver performs xenobiotic excretion out of hepatocytes with metabolic function. However, hepatocellular metabolism was non-uniform in hepatocyte. Hepatocellular metabolism could be different in nucleus and cytoplasm. In this study, we use the molecular probe 6-carboxyfluorescein diacetate (6-CFDA) to simulate xenobiotic metabolism in hepatocytes with multi-photon fluorescence microscopy in vivo. 6-CFDA was processed by intracellular esterase to 6- carboxyfluorescein (6-CF) with green fluorescence. And this probe was used to study differences in cytoplasmic and nuclear metabolism of hepatocytes.

Paper Details

Date Published: 21 February 2017
PDF: 6 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691J (21 February 2017); doi: 10.1117/12.2254944
Show Author Affiliations
Chih-Ju Lin, National Taiwan Univ. (Taiwan)
Ning Kang, National Taiwan Univ. (Taiwan)
Jian-Ye Lee, National Taiwan Univ. (Taiwan)
Hung-Ming Lin, National Taiwan Univ. (Taiwan)
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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