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Proceedings Paper

Wide-field surface-enhanced CARS microscopy of cells (Conference Presentation)

Paper Abstract

We have previously demonstrated a total internal reflection, wide-field CARS microscope, where the signal is enhanced with the aid of a thin gold layer that supports surface plasmon polariton resonances. This surface-enhanced CARS microscope is capable of generating images of lipid structures in close proximity (<100 nm) to the glass substrate at excitation densities that are 4 orders of magnitude lower than in point-scanning CARS microscopy. In this contribution, we demonstrate its application to visualizing lipids in aqueous media, including imaging of cells, with a unique surface-sensitive contrast that cannot be obtained with conventional CARS microscopy.

Paper Details

Date Published: 24 April 2017
PDF: 1 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006910 (24 April 2017); doi: 10.1117/12.2254549
Show Author Affiliations
Alexander Fast, Univ. of California, Irvine (United States)
John T. Kenison, Univ. of California, Irvine (United States)
Eric O. Potma, Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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