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Proceedings Paper

A fast image registration approach of neural activities in light-sheet fluorescence microscopy images
Author(s): Hui Meng; Hui Hui; Chaoen Hu; Xin Yang; Jie Tian
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Paper Abstract

The ability of fast and single-neuron resolution imaging of neural activities enables light-sheet fluorescence microscopy (LSFM) as a powerful imaging technique in functional neural connection applications. The state-of-art LSFM imaging system can record the neuronal activities of entire brain for small animal, such as zebrafish or C. elegans at single-neuron resolution. However, the stimulated and spontaneous movements in animal brain result in inconsistent neuron positions during recording process. It is time consuming to register the acquired large-scale images with conventional method. In this work, we address the problem of fast registration of neural positions in stacks of LSFM images. This is necessary to register brain structures and activities. To achieve fast registration of neural activities, we present a rigid registration architecture by implementation of Graphics Processing Unit (GPU). In this approach, the image stacks were preprocessed on GPU by mean stretching to reduce the computation effort. The present image was registered to the previous image stack that considered as reference. A fast Fourier transform (FFT) algorithm was used for calculating the shift of the image stack. The calculations for image registration were performed in different threads while the preparation functionality was refactored and called only once by the master thread. We implemented our registration algorithm on NVIDIA Quadro K4200 GPU under Compute Unified Device Architecture (CUDA) programming environment. The experimental results showed that the registration computation can speed-up to 550ms for a full high-resolution brain image. Our approach also has potential to be used for other dynamic image registrations in biomedical applications.

Paper Details

Date Published: 13 March 2017
PDF: 6 pages
Proc. SPIE 10137, Medical Imaging 2017: Biomedical Applications in Molecular, Structural, and Functional Imaging, 101370T (13 March 2017); doi: 10.1117/12.2254403
Show Author Affiliations
Hui Meng, Key Lab. of Molecular Imaging (China)
Institute of Automation (China)
Hui Hui, Key Lab. of Molecular Imaging (China)
Institute of Automation (China)
Chaoen Hu, Key Lab. of Molecular Imaging (China)
Institute of Automation (China)
Xin Yang, Key Lab. of Molecular Imaging (China)
Institute of Automation (China)
Jie Tian, Key Lab. of Molecular Imaging (China)
Institute of Automation (China)


Published in SPIE Proceedings Vol. 10137:
Medical Imaging 2017: Biomedical Applications in Molecular, Structural, and Functional Imaging
Andrzej Krol; Barjor Gimi, Editor(s)

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