Share Email Print

Proceedings Paper

Microfluidic devices for stem-cell cultivation, differentiation and toxicity testing
Author(s): Holger Becker; Thomas Hansen-Hagge; Andreas Kurtz; Ralf Mrowka; Stefan Wölfl; Claudia Gärtner
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The development of new drugs is time-consuming, extremely expensive and often promising drug candidates fail in late stages of the development process due to the lack of suitable tools to either predict toxicological effects or to test drug candidates in physiologically relevant environments prior to clinical tests. We therefore try to develop diagnostic multiorgan microfluidic chips based on patient specific induced pluripotent stem cell (iPS) technology to explore liver dependent toxic effects of drugs on individual human tissues such as liver or kidney cells. Based initially on standardized microfluidic modules for cell culture, we have developed integrated microfluidic devices which contain different chambers for cell/tissue cultivation. The devices are manufactured using injection molding of thermoplastic polymers such as polystyrene or cyclo-olefin polymer. In the project, suitable surface modification methods of the used materials had to be explored. We have been able to successfully demonstrate the seeding, cultivation and further differentiation of modified iPS, as shown by the use of differentiation markers, thus providing a suitable platform for toxicity testing and potential tissue-tissue interactions.

Paper Details

Date Published: 28 February 2017
PDF: 5 pages
Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 1006116 (28 February 2017); doi: 10.1117/12.2254026
Show Author Affiliations
Holger Becker, microfluidic ChipShop GmbH (Germany)
Thomas Hansen-Hagge, microfluidic ChipShop GmbH (Germany)
Andreas Kurtz, Charité Universitätsmedizin Berlin (Germany)
Ralf Mrowka, Univ. Clinics Jena (Germany)
Stefan Wölfl, Heidelberg Univ. (Germany)
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)

Published in SPIE Proceedings Vol. 10061:
Microfluidics, BioMEMS, and Medical Microsystems XV
Bonnie L. Gray; Holger Becker, Editor(s)

© SPIE. Terms of Use
Back to Top