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A three-photon microscope with adaptive optics for deep-tissue in vivo structural and functional brain imaging
Author(s): Xiaodong Tao; Ju Lu; Tuwin Lam; Ramiro Rodriguez; Yi Zuo; Joel Kubby
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Paper Abstract

We developed a three-photon adaptive optics add-on to a commercial two-photon laser scanning microscope. We demonstrated its capability for structural and functional imaging of neurons labeled with genetically encoded red fluorescent proteins or calcium indicators deep in the living mouse brain with cellular and subcellular resolution.

Paper Details

Date Published: 8 February 2017
PDF: 9 pages
Proc. SPIE 10051, Neural Imaging and Sensing, 100510R (8 February 2017); doi: 10.1117/12.2253922
Show Author Affiliations
Xiaodong Tao, Univ. of California, Santa Cruz (United States)
Ju Lu, Univ. of California, Santa Cruz (United States)
Tuwin Lam, Univ. of California, Santa Cruz (United States)
Ramiro Rodriguez, Univ. of California, Santa Cruz (United States)
Yi Zuo, Univ. of California, Santa Cruz (United States)
Joel Kubby, Univ. of California, Santa Cruz (United States)


Published in SPIE Proceedings Vol. 10051:
Neural Imaging and Sensing
Qingming Luo; Jun Ding, Editor(s)

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