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Proceedings Paper

New approaches to intracellular drug imaging by stimulated Raman scattering microscopy
Author(s): M. Lee; W. J. Tipping; A. Serrels; A. N. Hulme; V. G. Brunton
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Paper Abstract

A range of proposed alkyne Raman tags are examined in-silico for activity and then synthesised generating a library of analogues of the natural product, anisomycin. We report the use of bisaryl butadiyne-anisomycin (BADY-anisomycin) in intracellular SRS microscopy studies of uptake and localisation within live and fixed cells. Following rational design and synthesis, BADY-anisomycin was shown to produce an intense Raman band at 2219 cm-1, that is centrally located within the cellular silent region and is approximately 60 times more Raman active than the corresponding propargylanisomycin. Finally, we demonstrate two-colour imaging utilising EdU, an alkyne-containing proliferation probe and BADY-anisomycin.

Paper Details

Date Published: 21 February 2017
PDF: 8 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690T (21 February 2017); doi: 10.1117/12.2253847
Show Author Affiliations
M. Lee, The Univ. of Edinburgh (United Kingdom)
W. J. Tipping, The Univ. of Edinburgh (United Kingdom)
A. Serrels, The Univ. of Edinburgh (United Kingdom)
A. N. Hulme, The Univ. of Edinburgh (United Kingdom)
V. G. Brunton, The Univ. of Edinburgh (United Kingdom)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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