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Proceedings Paper

Light-induced quantitative microprinting of biomolecules
Author(s): Pierre-Olivier Strale; Ammar Azioune; Ghislain Bugnicourt; Yohan Lecomte; Makhlad Chahid; Vincent Studer
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Paper Abstract

Printing of biomolecules on substrates has developed tremendously in the past few years. The existing methods either rely on slow serial writing processes or on parallelized photolithographic techniques where cumbersome mask alignment procedures usually impair the ability to generate multi-protein patterns. We recently developed a new technology allowing for high resolution multi protein micro-patterning. This technology named “Light-Induced Molecular Adsorption of Proteins (LIMAP)” is based on a water-soluble photo-initiator able to reverse the antifouling property of polymer brushes when exposed to UV light. We developed a wide-field pattern projection system based on a DMD coupled to a conventional microscope which permits to generate arbitrary grayscale patterns of UV light at the micron scale. Interestingly, the density of adsorbed molecules scales with the dose of UV light thus allowing the quantitative patterning of biomolecules. The very low non specific background of biomolecules outside of the UV-exposed areas allows for the sequential printing of multiple proteins without alignment procedures. Protein patterns ranging from 500 nm up to 1 mm can be performed within seconds, as well as gradients of arbitrary shapes. The range of applications of the LIMAP approach extends from the single molecule up to the multicellular scale with an exquisite control over local protein density. We show that it can be used to generate complex protein landscapes useful to study protein-protein, cell-cell and cell-matrix interactions.

Paper Details

Date Published: 20 February 2017
PDF: 8 pages
Proc. SPIE 10117, Emerging Digital Micromirror Device Based Systems and Applications IX, 101170B (20 February 2017); doi: 10.1117/12.2253831
Show Author Affiliations
Pierre-Olivier Strale, Univ. of Bordeaux, Interdisciplinary Institute for Neuroscience, CNRS (France)
Ammar Azioune, Univ. of Bordeaux, Interdisciplinary Institute for Neuroscience, CNRS (France)
Ghislain Bugnicourt, Univ. of Bordeaux, Interdisciplinary Institute for Neuroscience, CNRS (France)
Yohan Lecomte, Univ. of Bordeaux, Interdisciplinary Institute for Neuroscience, CNRS (France)
Makhlad Chahid, Univ. of Bordeaux, Interdisciplinary Institute for Neuroscience, CNRS (France)
Vincent Studer, Univ. of Bordeaux, Interdisciplinary Institute for Neuroscience, CNRS (France)


Published in SPIE Proceedings Vol. 10117:
Emerging Digital Micromirror Device Based Systems and Applications IX
Michael R. Douglass; Benjamin L. Lee, Editor(s)

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